KOVAC'S REAGENT STRIPS

The various enzymes involved in the degradation of tryptophan to indole are collectively called as tryptophanase, a
general term used to denote the complete system of enzymes. The presence of indole is detected by the Kovac’s
reagent strip which turns pink in the presence of indole. Kovac’s Reagent Strips are sterile filter paper strips
impregnated with Kovac’s reagent. Peptone is used in the preparation of Peptone Water because of its high tryptophan
content. When tryptophan is degraded by bacteria, indole is produced. Tryptone Water can also be used to detect
indole production in the identification of members of coliform group.

For indole testing.