LEAD ACETATE AGAR

Salmonella, Shigella, Yersinia species and certain strains of Escherichia coli cause severe gastroenteritis and life- threatening systemic illness in human. Of these, Salmonella Typhi can be differentiated due to their ability to form hydrogen sulphide.
Lead Acetate Agar is the modification of the original formulation of Spray. This medium was successfully used to study hydrogen sulphide production. Lead Acetate Agar can also be used to differentiate between Salmonella Paratyphi A and Salmonella Paratyphi B. The latter produces hydrogen sulphide, observed as browning of the medium, within 18-24 hours, whereas the former fails to produces hydrogen sulphide.

for detection of H2S producing enteric bacteria