WILLIS AND HOBB’S MEDIUM BASE (IS : 5887 (Part 4) : 1999)

Wills and Hobb?s Medium Base is recommended for the isolation & identification of Clostridium from foods. Clostridium spp. has the ability to produce gastrointestinal tract-active toxins that are capable of causing foodborne diseases. Their heat resistant property allows the spores to survive incomplete cooking thus leading to food poisoning. Therefore, it becomes very important for these pathogens to be detected and isolated before the consumption of food. This medium is formulated by Willis and Hobbs and is used for the identification of C.perfringens on the basis of lecithinase reaction in egg yolk and lactose fermentation. This medium is prepared in accordance with Indian Standard under the specifications IS: 5887 (Part-IV) 1976.

for isolation and identi?cation of Clostridium from foods.