ANTIBIOTIC SULPHONAMIDE SENSITIVITY TEST AGAR (ASS AGAR)

Ericsson and Sherris on behalf of the German Institute of Standardisation and World Health Organization (WHO) developed an accurate quantitative method for antibiotic sensitivity testing. WHOs Expert Committee on Antibiotics have set certain requirements to be fulfilled by Sensitivity Test Agar. Antibiotic Sulphonamide Sensitivity Test Agar (ASS Agar) fulfils these criteria. This media can be used for detecting the presence of antimicrobial substances in milk, urine and other fluids as cited by Ansorg and Sogard. The presence of various amino acids makes the media favourable for growth and testing of various fastidious organisms like Listeria, Streptococci and Neisseria etc. The medium constituents do not inhibit the growth of the test organism. Therefore, the zones of inhibition obtained are solely due to the antibiotic used. Standard Methods are employed for sensitivity testing.

for testing antimicrobial activity of antibiotics and sulphonamides and also for detecting the presence of antimicrobial substances