BUFFERED PEPTONE WATER (as per EP)

Buffered Peptone Water is recommended by the European Pharmacopoeia for microbiological examination of non ? sterile products. It is used in the preparation of the samples to dissolve or dilute water-soluble products (1:10 dilution) and non-fatty products insoluble in water (in general 1:10 dilution, but some may require larger volumes of Buffered Peptone Water) Fatty products are homogenized with a suitable sterile surface-active agent such as polysorbate or tween 80 heated if necessary to no more than 40?C or, in exceptional cases, to no more than 45?C. They are mixed carefully and if necessary the temperature is maintained in a water-bath. It is added enough of the pre-warmed Buffered Peptone Water diluent to make a 1:10 dilution of the original product.

for pre enrichment of injured Salmonella species prior to selective enrichment and isolation