KING’S MEDIUM B BROTH

Pseudomonas aeruginosa is known to produce two types of pigments, pyocyanin and fluorescein which is a characteristic property and aids in isolation of Pseudomonas from clinical material. An additional pigment called as pyorubin was reported by King. Pyocyanin is green while fluorescein is fluorescent yellow and pyorubin is reddish brown. Some strains produce all these pigments while the others produce one or two pigments. King?s Medium B is suited for fluorescein. This medium can be used as a general medium for the non-selective isolation and pigment production of Pseudomonas species from foods, cosmetic samples etc. Agrobacterium have been traditionally identified as Gram-negative bacteria that do not produce fluorescent pigment on King?s B medium and do produce tumors (or hairy roots) when inoculated onto test plants.

for detection of pigment production by Pseudomonas species