ANTIBIOTIC ASSAY MEDIUM E

Antibiotic Assay Medium E is widely used as seed agar in the plate assay of Framycetin sulphate and Neomycin sulphate using Bacillus subtilis and Bacillus pumilus as test organism. This medium is formulated in accordance to British Pharmacopoeia and European Pharmacopoeia.
Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterile seed agar cooled to 40-45?C and spread evenly over the surface of solidified base agar. Zones of inhibition around the antibiotic are then measured. All conditions in the microbiological assay must be controlled carefully. The use of standard culture media in the test is one of the important steps for good results.

used in the microbiological assay of Neomycin sulphate and Framycetin sulphate using Bacillus
subtilis and Bacillus pumilus