FLUID TETRATHIONATE MEDIUM W/O IODINE AND BG, MODIFIED

Salmonellosis is a disease caused by Salmonella present in raw or undercooked food. Salmonella is the most common bacterial form responsible for food poisoning causing diarrhoea, vomiting, abdominal cramps and fever lasting for 4-7 days. It can cause serious illness due to consumption of such foods in older adults, infants and persons with chronic diseases. These are present in small numbers compared to coliforms that necessitates the examination of a relatively large sample for the isolation of organism. Due to processing of foods by physical and mechanical methods, sublethally injured Salmonella species are often present in foods and needs to be enumerated by resuscitating in suitable enrichment medium.
Enrichment medias include Rappaport Vassilidias medium and Fluid Tetrathionate Broth w/o Iodine and BG, Modified. It is a second selective enrichment medium recommended by FDA, BAM for enriching such Salmonella species from food samples under study. Fluid Tetrathionate Medium was originally devised by Mueller for enrichment of Salmonella. Organisms possessing the enzyme tetrathionate reductase are known to grow in this media.

for the selective enrichment method for isolating Salmonellae from food and other materials of sanitary importance in accordance with FDA BAM, 1998