MIDDLEBROOK 7H9 BROTH BASE

Media for Mycobacterial cultivation may be egg-based (Lowenstein Jensen Media) or agar-based (Middlebrook Media). Dubos and Middlebrook developed various formulations containing oleic acid and albumin, which protect Mycobacterium from toxic agents, helping for the growth of tubercle bacilli. Middlebrook 7H9 Broth Base was formulated by Middlebrook and Middlebrook et al and Schaeffer. This medium with Middlebrook ADC Growth Supplement and glycerol or polysorbate 80 is also recommended for cultivation of Mycobacteria and for assaying the INH content of the patient?s sera. The medium can also be used for preparing inocula for antimicrobial assays, as a basal medium for biochemical tests and for the subculture of stock strains.
Mycobacteria are strict aerobes and therefore increased CO2 tension and aerobic conditions must be provided during incubation. Care should be taken while decontamination of the specimen. Also proper specimen collection (sputum and not saliva) should be ensured. Samples should be carefully handled to avoid contamination.

for cultivation and sensitivity testing of Mycobacterium tuberculosis