M9 MINIMAL MEDIUM SALTS (5X)

M9 Minimal Salts, 5x is used in preparing M9 Minimal medium used for the cultivation and maintenance of E. coli in molecular biology. M9 is a chemically defined minimal growth medium for E. coli cultures. It can be supplemented with specific amino acids or other required nutrients for the selection of some specific auxotroph. Sometimes the incorporation of certain additives (e.g. thiamine or casamino acids) enhances the bacterial growth. In 1959, Jacob and Monod published an article where they discussed about minimal media of E. coli.

for growing Escherichia coli for molecular biology purpose