Product Code TM 2193
Description
Burman substituted bile salts with teepol in Membrane Enriched Teepol Broth, the membrane filtration test medium used to detect coliform organisms in water. M-Lauryl Sulphate Agar is prepared by substituting teepol with sodium lauryl sulphate.
The water samples are filtered through sterile membrane filter and then placed face upward on agar plates contains M-Lauryl Sulphate Agar. Burman recommended the following incubation temperatures and durations.
Unchlorinated waters:
Coliform organisms :4 hours at 30?C followed by 14 hours at 35?C
Escherichia coli: 4 hours at 30?C followed by 14 hours at 44?C
Non-chlorinated organisms benefit from 4 hours? incubation at 30?C but chlorinated organisms require 6 hours incubation at 25?C. After incubation, yellow colonies are formed which should be confirmed further.
Principle
for enumeration of Escherichia coli and coliforms in water, using membrane ?lter technique
Microorganism
Industry
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