M-STAPHYLOCOCCUS BROTH

The swimming pool water is generally potable and treated with additional disinfectants but it also may come from thermal springs or salt water. Modern pools have a recirculation system for filtration and disinfection. Staphylococci are gram-positive cocci residing on the skin and mucous membrane of humans and other organisms.
M-Staphylococcus Broth is used for detection and isolation of Staphylococci by membrane filter technique. This broth is especially used for isolating pathogenic and enterotoxigenic Staphylococci and gelatin.
Inoculate the tubes of M-Staphylococcal Broth and incubate at 35 ? 2?C for 24 hours. Streak from positive tubes (turbid growth) on plates of Lipovitellin Salt Mannitol Agar Base and incubate at 35-37?C for 48 hours. Opaque, yellow zones around the colonies are positive evidence of lipovitellin- lipase activity and mannitol fermentation. Alternatively, around 2 ml of M-Staphylococcus Broth is used to saturate sterile absorbent cotton pads. Membrane filters used for filtration are aseptically placed on these saturated cotton pads. Following an incubation at 35-37?C for 18-48 hours, observe membrane for growth and pigment production. Mannitol fermentation can be visualized as yellow colouration by addition of a few drops of bromothymol blue to the areas from where colonies have been removed.

for detection and isolation of Staphylococci by membrane ?lter technique