MacCONKEY AGAR (W/ 0.075 NR, 0.5% BILE SALTS & 1.2% AGAR W/O CV & NaCl)

MacConkey Agar is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of clinical specimens. Subsequently MacConkey Agar was recommended for use in microbiological examination of foodstuffs and for direct plating / inoculation of water samples for coliform counts. These media are also accepted by the Standard Methods for the Examination of Milk and Dairy Products and pharmaceutical preparations. The original medium contains peptone, bile salts, sodium chloride and two dyes. MacConkey Agar w/o CV, NaCl W/ 0.5% Bile Salts is a modification of the original formulation with the exception of crystal violet and sodium chloride. This medium prevents the swarming of Proteus species that are generally encountered in pathological specimens. Also potentially pathogenic gram- positive organisms can be isolated using this medium.

for isolation and di?erentiation of lactose fermenting and nonlactose fermenting enteric bacteria