SELENITE CYSTINE BROTH BASE W/O BISELENITE

Klett first demonstrated the selective inhibitory effects of selenite and Guth used it to isolate Salmonella Typhi. Leifson fully investigated selenite and formulated the media. Selenite Cystine Medium is a modification of Leifsons formula with added cystine. Modification of original composition and similar media are recommended by AOAC, APHA, USP etc. Enrichment media are routinely employed for detection of pathogens in faecal specimens as the pathogens are present in a very small number in the intestinal flora. Selenite Cystine Broth is useful for detecting Salmonella in the nonacute stages of illness when organisms occur in the faeces in low numbers and for epidemiological studies to enhance the detection of low number of organisms from asymptomatic or convalescent patients.

for selective enrichment of Salmonella spp. and possibly Shigella sonnei from faeces, urine, water and foodstu?s