PEPTONE WATER

Peptone Water is particularly suitable as a substrate in the study of indole production. Peptone used in Peptone Water is rich in tryptophan content. Presence of indole can be demonstrated using either Kovacs or Ehlrich reagent. Peptone Water is also utilized as a base for carbohydrate fermentation studies with the addition of sugar and indicators such as bromocresol purple, phenol red or bromothymol blue. Peptone Water is recommended for studying the ability of an organism to ferment a specific carbohydrate which aid in differentiation of genera and species. Peptone water is formulated as per Shread, Donovan and Lee. Peptone Water with pH adjusted to 8.4 is suitable for the cultivation and enrichment of Vibrio species.