GLUCOSE YEAST PEPTONE AGAR

Yeasts are unicellular organisms that reproduce by budding. Their microscopic and morphological features usually appear similar for different genera and are not particularly helpful in their isolation in pure culture. Glucose Yeast Peptone Agar is formulated as described by Subba Rao with a slight modification in agar concentration for isolating yeasts from soil specimens. This is a highly nutritious medium, which may be used not only for isolating yeasts but also for isolating some fastidious microorganisms. Yeasts grow well on a minimal medium containing only dextrose and salts. The addition of protein and yeast cell extract hydrolysates allows faster growth so that during exponential or log-phase growth, doubling time of 90 minutes is observed.

for isolation of yeasts from soil samples