CLOSTRIDIAL AGAR

One of the major species of anaerobic bacteria to cause disease in humans is Clostridium. Clostridium species cause tetanus and gas gangrene that ultimately leads to tissue damage. Another Clostridium species produces the lethal botulinum toxin, the causative agent of botulism. Clostridial Agar formulated by Vera is recommended for the selective isolation of pathogenic Clostridia form mixed flora. The media is well supplemented to support luxuriant growth of Clostridium species.
Accompanying enteric bacteria including Proteus, Pseudomonas and Bacillus species are inhibited by neomycin sulphate and sodium azide incorporated in the medium. The ideal method of inoculation of Clostridial Agar is direct inoculation of sterile, cooled medium with the specimen (in tubes). Alternatively, agar plates of the medium can also be inoculated by streaking.

for selective isolation of pathogenic Clostridia from mixed ?ora