DUBOS OLEIC BROTH BASE

Tuberculosis remains a major global public health problem worldwide. Mycobacterium tuberculosis, the causative agent of tuberculosis in man, is carried in airborne particles known as droplet nuclei that are generated when patients with pulmonary tuberculosis cough. Infections occur when a susceptible person inhales the droplet nuclei containing the bacterium. Dubos Oleic Broth Base is recommended by Dubos and Middlebrook for the primary isolation and subsequent cultivation of the tubercle bacilli. On comparative studies of various media, Dubos Oleic Agar Base was found to be superior to other media for the primary isolation of Mycobacteria. Mycobacteria grow very rapidly when inoculated in a broth media and therefore preliminary culture of all the test samples in a broth media is recommended.

for cultivation of Mycobacterium tuberculosis