L. D. AGAR

Organisms that grow in the absence of oxygen are termed as anaerobes. Depending upon their ability to tolerate oxygen, they are classified as either facultative or obligate anaerobes. The anaerobic gram-negative bacteria are part of the normal flora of the upper respiratory tract, mouth, intestinal tract and urinogenital tract of human and animals. The bile-resistant Bacteroides fragilis group is the most commonly recovered anaerobe in clinical specimens and is more resistant to antimicrobial agents than any other anaerobe. Fusobacterium necrophorum is a very virulent anaerobe that may cause severe infections, usually in children or young adults.
L. D. Medium or Lombard-Dowell Medium was developed by Dowell and Lombard for the cultivation and identification of fastidious anaerobic bacteria. L. D. Agar is used to evaluate the degree of growth of anaerobes and also to assess indole and catalase production by Bacteroides and Fusobacterium species.

for identi?cation and cultivation of fastidious anaerobic bacteria