WILKINS CHALGREN ANAEROBIC BROTH BASE

Wilkins Chalgren Anaerobic Broth Base, formulated by Wilkins and Chalgren, is the preferred medium for susceptibility testing of anaerobes. This medium is also recommended for testing anaerobic bacteria. Wilkins Chalgren Anaerobic Broth Base is similar to the agar medium, except the agar. The broth medium is especially useful in the broth micro-dilution tests. Wilkins Chalgren Broth media need to be appropriately supplemented to support the growth of certain anaerobic bacteria. Hemin and Menadione (Vitamin K3) enhances the growth of Bacteroides species and Prevotella melaninogenica, respectively and many other species of gram-negative anaerobic rods. The medium can also be supplemented with defibrinated or lysed blood for the growth of fastidious anaerobic bacteria.

for cultivation and susceptibility testing of anaerobic bacteria