VIBRIO PARAHAEMOLYTICUS SUCROSE AGAR (as per APHA) (VPSA) (VEG).

This medium is prepared by using vegetable peptones in place of animal based peptones which makes the medium free of BSE/TSE risks. Vibrio Parahaemolyticus Sucrose Veg Agar is the modification of Vibrio Parahaemolyticus Sucrose Agar (VPSA) which is recommended by APHA for isolating and enumerating Vibrio parahaemolyticus from sea foods. It is a differential medium (and also selective to some extent) which distinguishes Vibrio parahaemolyticus from other marine Vibrios species.
Suspected seafood sample when diluted and blended with sterile Veg Peptone Tween Salt Diluent (prepared by dissolving 1.0gm of Veg Peptone and 10.0gm of Tween 80 in 1 litre of distilled water and autoclaved at 121?C for 15 mins), is filtered through HGMF using sterile diluent as a carrier. HGMF is then aseptically transferred to the Tryptone Soya Veg Agar w/ Magnesium Sulphate plates and incubated for 4 hours at 35?C. HGMF is then transferred from TSAMS to the dry VPS Veg Agar plate and incubated for 18 – 20 hours at 42?C.

for isolation and enumeration of Vibrio parahaemolyticus from seafood