MIO MEDIUM (MOTILITY INDOLE ORNITHINE MEDIUM) (VEG.)

MIO Veg Medium is prepared by using vegetable peptones which are free of BSE/TSE risks. MIO Veg Medium is the modification of MIO Medium which was formulated by Ederer and Clark and Oberhofer and Hajkowski for detection of motility, indole and ornithine decarboxylation in single culture tube.
Organisms possessing ornithine decarboxylase, decarboxylates ornithine to putrescine which increases the pH making it alkaline, indicated by colour change from yellow to purple throughout the medium. Decarboxylase negative reaction is indicated by yellow colour or yellow with a purple band near the top of the medium. Indole is produced from tryptophan present in Veg hydrolysate. The indole produced combines with the aldehyde present in the Kovac?s reagent to form a red complex.

for identi?cation of Enterobacteriaceae on the basis of motility, indole production and ornithine decarboxylase activity