C.L.E.D. AGAR (W/ BROMO THYMOL BLUE) (BROLACIN AGAR) (VEG.)

These media are prepared by using vegetable peptones in place of animal peptones which are free from BSE/TSE risks. These media are the modification of C.L.E.D. Agar as devised by Mackey and Sandy. The original experiments and trials to control the swarming of Proteus led to formulate C.L.E.D. (Cystine-Lactose-Electrolyte-Deficient) medium which contained L-Cystine promoting growth of coliforms. Lactose is incorporated serving as an immediate readily available carbon source required for growth of urinary pathogens. Incorporation of pH indicator bromothymol blue helps in identifying lactose fermenting colonies. In case of very low pH of urine, around 5.0, a low bacterial count is often reported. This medium does not support growth of Shigella species.

for isolation and di?erentiation of urinary pathogens by lactose fermentation