ANTIBIOTIC ASSAY MEDIUM NO. 5 (STREPTOMYCIN ASSAY AGAR W/ YEAST EXTRACT) (as per IP/ USP)

This medium is commonly used for assaying Streptomycin by cylinder plate method using Bacillus subtilis as test organism. This method is used in the assay of commercial preparations of antibiotics, as well as for antibiotics in body fluids, feeds etc. Medium composition is in accordance to the specifications detailed in the FDA and numerically identical to the name assigned by Grove and Randall.
This medium is used to prepare the base as well as seed layer in the microbiological assay of antibiotics such as Dihydrostreptomycin, Framycetin and Kanamycin B.
To perform the antibiotic assay the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized test culture can be overlaid. Even distribution of the layer is important.

for microbiological assay of Streptomycin using Bacillus subtilis