ANTIBIOTIC ASSAY MEDIUM NO. 13 (NYSTATIN ASSAY BROTH)

This medium is formulated in accordance to CFR and is numerically identical with the name assigned by Groove and Randall. Schmidt & Moyer has reported the use of antibiotic assay medium for liquid formulation in performance of antibiotic assay. This medium is widely used in turbidometric assay of antifungals like candicidin using test organism like Saccharomyces cerevisiae. The is medium is also termed Sabouraud Liquid Broth Modified or Fluid Sabouraud Medium. Turbidimetric antibiotic assay is based on the change or inhibition of growth of a test microorganism in a liquid medium containing a uniform concentration of an antibiotic.

for microbiological assay of Candicidin using Saccharomyces cerevisiae