MYCOPLASMA SYNOVIAE MEDIUM BASE

The two Mycoplasma Species Mycoplasma hominis and Ureaplasma urealyticum, have been implicated in urogenital infections in men and women. The organisms are also much smaller than most bacteria measuring 0.2 to 0.3 ?m, hence they are able to pass through bacteriological filters. These organisms differ from other bacteria in that they lack a rigid cell wall. Individual cells are bound only by trilaminae unit membrane. Thus cultivation of Mycoplasma and Ureaplasma requires an enriched medium containing precursors for nucleic acid, protein and lipid biosynthesis. Precursors for nucleic acids and proteins are provided principally by the enriched basal peptone medium and yeast extract, while lipids are provided by the inclusion of serum. In fact, one of the principle criteria used in the taxonomic classification of these organisms is the requirement for the complex lipid cholesterol in the growth medium by certain Mycoplasma and Mycoplasma-like organisms. Urogenital Mycoplasma Broth Base (Mycoplasma Urogenital Broth Base) is based on the formula used by Bebear et al, Fiacco et al, Bonissol and Daoulas, Renaudin et al and Bauriaud et al. This medium is used for selective cultivation of urogenital Mycoplasma, viz. M. hominis and U. urealyticum from clinical samples.

for selective isolation of Mycoplasma hominis and Ureaplasma urealyticum