BHI W/ 0.1% AGAR (BRAIN HEART INFUSION W/ 0.1% AGAR)

BHI Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. Brain Heart Infusion Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth. Brain Heart Infusion Broth is also the preferred medium for anaerobic bacteria, yeasts and moulds. This medium is nutritious and well buffered to support the growth of wide variety of organisms. With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other fungi. Agar in 0.1% concentration improves growth of microaerophillic and anaerobic microorganisms. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended

for propagation of fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations