CHROMOGENIC L. MONO DIFFERENTIAL AGAR BASE

Listeria monocytogenes is a gram-positive foodborne human pathogen responsible for serious infections in pregnant women that may ultimately result in abortion, stillbirth, birth of a child with neonatal listeriosis and meningitis or primary bacteremia in adults and juveniles. The pathogenicity of Listeria ivanovii for humans is uncertain. Since L. monocytogenes nd L. innocua have similar biochemical properties, they cannot be differentiated on traditional media (PALCAM, Oxford). Chromogenic L.mono Differential Agar Base is based on for the selective and differential isolation of Listeria species on the basis of utilization of chromogenic substrate and lecithinase activity [Phosphotidylcholine phospholipase C (PCPLC)] .PI-PLC and PC-PLC, the major virulence factors, are only produced by pathogenic L. monocytogenes and Listeria ivanovii.

for the selective and di?erential isolation, enumeration and identi?cation of Listeria monocytogenes and Listeria species based on PCPLC activity