ALTERNATIVE THIOGLYCOLLATE MEDIUM (NIH THIOGLYCOLLATE MEDIUM) (as per IP)

NIH Thioglycollate Medium is formulated as described in the N.I.H. memorandum, Indian Pharmacopoeia. This medium is used for sterility testing for detecting the presence of viable forms of microorganisms in or on pharmaceutical preparations. This medium is also used for sterility checking for devices having tubes with small lumina. NIH Thioglycollate Medium is generally used for products containing mercurial preservatives when the oxidation reduction indicator is not present or required. Lack of an indicator in the medium avoids possible toxicity to organisms. NIH Thioglycollate Medium contains sodium thioglycollate that can neutralize the bacteriostatic effect of mercurial preservatives. Absence of agar makes it suitable for testing viscous materials and devices having tubes with small lumina.

for sterility testing of turbid or viscous biological products