ANTIBIOTIC ASSAY MEDIUM NO. 36 (SOYA BEAN CASEIN DIGEST AGAR) (TRYPTONE SOYA AGAR) (as per USP)

Soyabean Casein Digest Agar is a widely used medium, which supports the growth of wide variety of organisms even that of fastidious ones such as Neisseria, Listeria, and Brucella etc. The medium with addition of blood provides perfectly defined haemolysis zones, while preventing the lysis of erythrocytes due to its sodium chloride content. It has been frequently used in the health industry to produce antigens, toxins etc. It’s simple and inhibitor-free composition makes it suitable for the detection of antimicrobial agents in the food and other products. Tryptone Soya Agar is recommended by various pharmacopoeias as sterility testing medium.
Tryptone Soya Agar conforms as per USP and is used in microbial limit test and antimicrobial preservative – effective test. Gunn et al used this medium for the growth of fastidious organisms and study of haemolytic reaction after addition of 5%v/v blood.

for enrichment and isolation of fastidious microorganisms with or without blood