V.C.N. SUPPLEMENT

Rehydrate the contents of 1 vial aseptically with 2 ml sterile distilled water. Mix well and aseptically add it to TM 933 –
THAYER MARTIN MEDIUM BASE for 440 ml of medium aseptically add 50ml sterile lysed blood and one vial of V.C.N.
Supplement along with one vial of Vitamino Growth Supplement, 2% Haemoglobin Solution (250ml) can be used instead
of sterile lysed blood in 250ml of medium.
In TM 116 – GC AGAR BASE / TMV 116 – GC AGAR BASE (VEG.) for 250 ml of can be used instead of sterile lysed blood in
250 ml of 2% Haemoglobin Solution and GC Supplement w/Antibiotics, one vial of Vitamino Growth Supplement w/
Antibiotics for additional selectivity. If desired V.C.N. Supplement can be used along with GC Supplement w/ Antibiotics
for additional selectivity.
In TM 1105 – TRANSGROW MEDIUM BASE for 440 ml of medium aseptically add 50 ml of sterile 2% Haemoglobin Solution
and one vial of V.C.N. Supplement along with one vial of Vitamino Growth Supplement.

For selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis.