AK AGAR NO. 2 (SPORULATING AGAR) (ARRET AND KIRSHBAUM MEDIUM)

AK Agar formulated by Arret and Kirshbaum is used for the production of spores of Bacillus subtilis ATCC 6633, which is used in the Penicillin Milk Test procedure. Detection of penicillin and other antibiotic residues in milk is of primary importance in the dairy industry. This is done by the Penicillin Milk Test procedure.
A fresh slant culture of Bacillus subtilis is washed with sterile physiological saline onto the surface of Roux bottles containing 300 ml sterile medium. The bottles are incubated at 35?C for 5 days and the resulting growth is suspended into 50 ml of sterile physiological saline. The growth is washed by centrifuging the suspension and discarding the supernatant. The sediment obtained is re-suspended in fresh sterile saline and heated at 70?C for 30 minutes to kill vegetative cells and obtain the spore suspension. This spore suspension can be stored for months for use in detection of penicillin/ antibiotic residues in milk and dairy products.

for production of spores of Bacillus subtilis ATCC 6633 which are used as inoculum in detection of penicillin and other antibiotic residues in milk and other dairy product