ANTIBIOTIC ASSAY MEDIUM F

Grove and Randall have elucidated those antibiotic assays and media in their comprehensive treatise on antibiotic assays. Antibiotic assay Medium F is recommended for the microbiological assay of Nystatin and Amphotericin B using Saccharomyces cerevisiae and Candida tropicalis. It is recommended by European Pharmacopoeia and British Pharmacopoeia.
Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterile seed agar cooled to 40-45?C and spread evenly over the surface of solidified base agar. After incubation the concentration of the antibiotic being assayed is determined by measuring the zone of inhibition obtained, with that of reference standard antibiotic. All conditions in the microbiological assay must be carefully controlled. The use of standard culture media in the test is one of the important steps for good results.

for microbiological assay of Amphotericin B and Nystatin using Saccharomyces cerevisiae and Candida tropicalis