ANTIBIOTIC ASSAY MEDIUM L – AOAC

Antibiotic Assay Medium L is formulated in accordance with AOAC for the microbiological assay of Monensin in feeds, using Bacillus subtilis as the test organism. Use single inoculated agar layer. Optimum concentration of suspension of Bacillus subtilis, is determined before assay by preparing trial plates. Usually 0.5 ml suspension is used per 100 ml of seed agar, to obtain appropriate inhibition zones (17.5? 2.5 mm with 0.5?g/ml). For actual assay add appropriate amount of suspension to sterile, molten medium, mix and pour 6 ml into sterile Petri plate.

for microbiological assay of Monensin using Bacillus subtilis