ANTIBIOTIC ASSAY MEDIUM NO. 3 (ASSAY BROTH) (VEG.)

Antibiotic Veg Assay Medium No.3 is prepared using vegetable peptones in place of animal peptones, making the medium BSE, TSE risks free. Antibiotic Veg Assay Medium No.3, can be used for the same purpose of Antibiotics Assay Medium No.3, used for the assay of various antibiotics. Grove and Randall have elaborately elucidated the methods to perform these assays and various media used for that. Schmidt and Moyer have reported the use of antibiotic assay medium for the liquid formulation used in the performance of antibiotic assay. These media are also recommended by USP and FDA generally in the turbidimetric antibiotic assay of Amikacin, Bacitracin, Kanamycin, Streptomycin and other antibiotics.
Antibiotic Veg Assay Medium No. 3 (Assay Veg Broth) can also be used in the microbiological assay of different antibiotics in pharmaceutical and food products by the turbidimetric method. Ripperre et al reported that turbidimetric methods for determining the potency of antibiotics are inherently more accurate and more precise than agar diffusion procedure. Turbidimetric antibiotic assay is based on the change or inhibition of growth of a test microorganims in a liquid medium containing a uniform concentration of an antibiotic. After incubation of the test organism in the working dilutions of the antibiotics, the amount of growth is determined by measuring the light transmittance using spectrophotometer. The concentration of antibiotic is determined by comparing amounts of growth obtained with that given by the reference standard solutions. Use of this method is appropriate only when test samples are clear.

for microbiological assay of antibiotics