ANTIMYCOTIC SENSITIVITY TEST AGAR

Antimycotic Sensitivity Test Agar is recommended for testing the antimycotic activity by disc diffusion method (Sensitivity disc or MIC testing). The Clinical and Laboratory Standards Institute (CLSI) subcommittee on Antifungal Susceptibility Testing has established interpretative break point for three drugs and Candida spp. The M44-A document (approved standard) became available in 2004. Espinel-Ingroff suggested that easier test modification to CLSI methods are desirable. Several workers have used agar diffusion method as an alternative approch to the CLSI methods. Stiller et al observed a good correlation between MICs and growth inhibition zones for 5-FC. Pfaller et al found a fluconazole disk test to be comparable to the MIC test. Barry and Brown demonstrated good correlation between fluconazole disk test and MIC determined by either broth dilution or E Test. Espinel et al and Pfaller et al evaluated E Test and suggested that it correlates well with CLSI reference methods. However, this agreement was species and medium dependent and they suggested the need for further optimisation of medium formulation.

for testing antimycotic sensitivity by di?usion method using antimycotic sensitivity discs