APT AGAR

APT (All purpose Tween 80) Agar is formulated as per Evans and Niven for cultivation and maintenance of Lactobacilli. This medium is also used in the microbiological assay of thiamine. Lactobacillus forms a major part of lactic acid bacteria group which are abundant in nature. They convert lactose and other sugars to lactic acid and therefore are named as Lactobacillus. They are responsible for spoilage of foods like meat, dairy etc. However APT Agar can also be used for cultivation of heterofermentative lactic acid bacteria requiring high thiamine content. APT Agar is also used as a maintenance medium since it preserves the viability and sensitivity of Weissella viridescens ATCC 12706 (formerly Lactobacillus viridescens)

for cultivation of hetero-fermentative Lactobacilli and other organisms by extra thiamine content