AZIDE DEXTROSE BROTH

Enterococci are more resistant to chlorine in water, hence are better indicators of sewage pollution than Escherichia coli. Until 1984, members of the genus Enterococcus were classified as Group D Streptococci. Upon genomic DNA analysis, a separate genus status was provided to them. Azide Dextrose Broth is recommended by APHA for enumeration of faecal Streptococci by MPN technique. Azide Dextrose Broth was initially formulated by Rothe, Mullmann and Seligmann for quantitative determination of Enterococci in water, sewage, foods and other materials suspected of contamination with sewage. When large volumes of water samples are to be examined, double strength medium is used. Turbidity in tubes indicates presence of Enterococci, however, it should be further confirmed by inoculation in Ethyl Violet Azide Broth.

for detection of faecal Streptococci in water, sewage, food and other materials