AZOTOBACTER MEDIUM (MANNITOL)

Bacteria of the family Azotobacteraceae constitute the majority of heterotrophic free-living nitrogen fixing bacteria. Azotobacter is a genus of free-living diazotrophic bacteria which have the highest metabolic rate compared to any other microorganisms. Azotobacter have generated a good deal of interest in the scientific community because of their unique mode of metabolism, by which they can fix nitrogen aerobically.
Azotobacter Medium (Mannitol) is used for isolation and cultivation of mannitol positive Azotobacter species from soil. It is also useful for maintenance of Azotobacter species by adding extra 1% Mannitol to the medium as specified by the American Type Culture Collection.

for isolation, cultivation and enrichment of Azotobacter species