BREWER THIOGLYCOLLATE MEDIUM, MODIFIED (LINDEN THIOGLYCOLLATE MEDIUM) (VEG.)

These media are prepared by completely replacing animal based peptones with vegetable peptones. Brewer Thioglycollate Veg medium is the modification of Brewer Thioglycollate Medium prepared as per the original formula of Brewer. Brewer Thioglycollate Veg Medium Modified is a modification of Linden Thioglycollate Medium.
The uninoculated medium shows bluish green colour at the top indicating presence of oxygen in that part. Modified medium contains more thioglycollate and was recommended for sterility testing procedures. Organisms which ferment dextrose and lower the pH to critical levels may not survive in this medium after growth has taken place.
Note: if more than the upper one third layer acquires bluish-green colour (absorbs oxygen), the dissolved oxygen can be removed by heating the medium in free flowing steam for 5-10 minutes or in a water bath untill the green colour disappears, and the prepared medium should be stored in the dark till use.

for sterility testing of biological products & for isolation of aerobes and anaerobes