BRILLIANT GREEN AGAR BASE W/ PHOSPHATES

Salmonella species cause many types of infections, from mild self-limiting gastroenteritis to life threatening typhoid fever. The most common form of Salmonella disease is self-limiting gastroenteritis with fever lasting less than 2 days and diarrhoea lasting less than 7 days. Brilliant Green Agar Base w/phosphates is formulated as per the recommendation of Rijks Institute Voorde Volksgezondheld (National Institute for Public Health), Utrecht. It is also recommended by the ISO Committee, because of its improved performance with respect to recovery of smaller numbers of Salmonella species, inhibition of Escherichia coli, Proteus species and Pseudomonas species.
The medium can further be supplemented with sulphacetamide (1g/l) and sodium mandelate (0.25g/l) to inhibit contaminating microorganisms when the sample is suspected to contain large number of competing organisms along with Salmonella species.
Brilliant Green Agar w/Phosphates being highly selective is recommended to be used along with a less inhibitory medium to improve the chances of recovery. Often cultures are enriched in Selenite Cystine Broth or Tetrathionate Broth. These enriched cultures are then isolated simultaneously on Brilliant Green Agar Base, SS Agar, Bismuth Sulphite Agar and MacConkey Agar.

for selective isolation & cultivation of Salmonellae by inhibiting E.coli, Proteus and Pseudomonas species