BRUCELLA AGAR BASE, MODIFIED

This medium is formulated so as to support luxuriant growth of fastidious bacteria like Campylobacter and Brucella species. For selective isolation of Brucella species, antibiotic mixtures are incorporated into the base. Farrel and Robinson formulated a highly selective antibiotic medium. Ethyl violet and Circulin, which were recommended originally, are no longer used. When non-selective medium is required, Brucella Broth Base may be employed with the addition of serum only (i.e. without antibiotics).
It is suggested in case of broth medium that half the tubes be incubated in the normal atmosphere, and half in a 10% CO2 enriched atmosphere. Brucella species are highly infectious and so extreme care should be taken while handling. All presumptive anaerobic organisms must be further confirmed by the tests.

for cultivation of Campylobacter species