BRUCELLA AGAR BASE (VEG.)

Brucella Veg Agar Base is prepared by completely replacing animal based peptones with vegetable peptones, making the medium BSE/TSE risks free. Brucella are intracellular parasites that cause epizootic abortions in animals and septicemic febrile illness or localized infections of bone, tissue or organ systems in humans. Brucella species are highly fastidious and therefore require a nutrient rich medium to be able to grow. Also, Brucella species are highly infective and so extreme care should be taken while handling. Brucella Agar Base is used for the isolation and cultivation of Brucella species. Brucella Veg Agar Base is the modification of Brucella Agar Base and serves the same purpose. The basal medium can be also used for the isolation of Campylobacter (with addition of Camplylobacter Supplements) and other fastidious bacteria like Brucella, Streptococci, pneumococci, Listeria, Neisseria meningitides and Haemophilus influenza. Brucella Agar is also recommended by APHA for isolation of Brucella species from foods.
The medium can also be enriched with 5 % v/v sterile defibrinated horse blood. For selective isolation of Brucella species antibiotic mixtures are incorporated into the base. Swab specimens can be directly streaked on the plate. Liquid specimens can be inoculated by means of an inoculation loop. All presumptive anaerobic organisms must be further confirmed by additional tests.

for selective isolation and cultivation of Brucella or Campylobacter species