BRUCELLA SELECTIVE MEDIUM BASE

Brucellosis is a zoonotic disease with a domestic animal reservoir. It is an occupational disease of veterinarians, microbiologists, farmers etc. The route of infections is genital, nasopharyngeal, gastrointestinal, conjunctival, respiratory and through abraded skin. Brucellosis in humans has a variable incubation period, an insidious or abrupt onset and no pathognomic symptoms or signs. Brucella Agar was designed for cultivating Brucella species from diagnostic specimens. With the incorporation of blood or other nutritious substances, it facilitates the cultivation of variety of fastidious anaerobic organisms. However, Brucella Medium is supplemented with antibiotics to prevent overgrowth of other accompanying organisms. Brucella Agar Base w/ 1.0 % Dextrose was originally developed by Jones and Morgan for preparations of serum-dextrose-antibiotic medium used for the isolation and cultivation of Brucella species. Addition of antibiotics (as FD) makes the medium highly selective for Brucella species. Ethyl violet and circulin, which were recommended initially, are no longer used

for isolation and identi?cation of Brucella species