BUFFERED PEPTONE WATER W/ NaCl (as per IP)

Buffered Peptone Water is a pre-enrichment medium designed to help recovery of sub-lethally damaged Salmonellae before transfer to a selective medium. This pre-enrichment medium is free from inhibitors and is well buffered and provides conditions for resuscitation of the cells that have been injured by processes of food preservation. It was noted by Edel and Kampelmacher that sub-lethal injury to Salmonella may occur due to food preservation techniques involving heat, desiccation, high osmotic pressure, preservatives or pH changes. Buffered Peptone Water during the pre-enrichment period helps in recovery of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens, which have low buffering capacity. These media can be used for testing dry poultry feed. In a survey involving isolation of Salmonellae from meat that had been artificially contaminated with sub-lethally injured organisms. Pre-enrichment in Buffered Peptone Water at 37?C for 18 hours before selection in Tetrathionate Brilliant Green Bile Broth showed superior results compared with direct selection method.
Lactose Broth is frequently used as a pre-enrichment medium but it may be detrimental to recovery of Salmonellae. The composition of Buffered Peptone Water with NaCl medium is as per IP and EP specifications recommended to dilute the sample for microbial examination. Depending on the amount of fat in the sample to examine the kind and quantity of emulsifying agent to be used.
Inoculate 10 grams of specimen in 50 ml of these media and incubate at 35-37?C for 18 hours. Transfer 10 ml from this medium to 100 ml of Tetrathionate Broth and incubate at 43?C for 24-48 hours and then subculture on selective plating media. Examine the plates for characteristic Salmonella colonies.

used as diluents for carrying out microbial limit test