BUFFERED YEAST AGAR

Yeasts grow well on a minimal medium containing only dextrose and salts. The addition of yeast extract allows faster growth so that during exponential or log phase growth, the cells divide every 90 minutes. Buffered Yeast Agar is prepared as per the modification of the yeast-salt medium described by Davis. The reaction of this medium can be adjusted to required pH values by the addition of citric or lactic acid to the medium after sterilization. The following table shows the amount of the acids required to be added to 100 ml of Buffered Yeast Agar cooled to 50?C.

for cultivation of yeasts and molds in brewery