C.L.E.D. AGAR BASE W/O INDICATOR

On a solid medium, Sandy’s reported that swarming of Proteus species could be controlled by restricting the electrolytes. Formerly swarming of Proteus was controlled by adding alcohol, surface-active agent, sodium azide, boric acid etc. to the medium. Later on Sandys medium was modified by Mackey and Sandy’s, by replacing mannitol by lactose and sucrose and elevating concentration of agar and bromothymol blue. This formulation was further modified by the same authors and called C.L.E.D. (Cystine-Lactose-Electrolyte-Deficient) by deleting the sucrose and by including L-cystine for promoting the growth of cystine dependent dwarf coliform colony. This medium is recommended for use in urine bacteriology, promoting the growth of all urinary pathogens. C.L.E.D. Medium is also recommended for dipstick procedures and as dip inoculum transport medium for urine specimens.
Bacteriuria may be quantitated by inoculating the surface of an agar medium by proper dilution. Inoculate the medium immediately after urine collection. It can also be inoculated by calibrated loop or duplicate dilution pour plate methods. Shigella species may not grow on this medium. Initiation of antibiotic therapy, before collection sample, low urine pH (less than 5) etc. may result in low bacterial count from infected patients.

for isolation, enumeration and presumptive identi?cation of bacterial ?ora in the urinary tract