C.L.E.D. AGAR (W/ BROMO THYMOL BLUE) (BROLACIN AGAR)

On a solid medium, Sandys reported that swarming of Proteus species can be controlled by restricting the electrolytes. Formerly swarming of Proteus was controlled by adding alcohol, surface-active agent, sodium azide, boric acid etc. to the medium. Later on Sandy’s medium was modified by Mackey and Sandys, by replacing mannitol by lactose and sucrose and elevating concentration of agar and bromothymol blue. This formulation was further modified by the same authors, called C.L.E.D. (Cystine-Lactose-Electrolyte-Deficient) by deleting the sucrose and by including L-cystine for promoting the growth of cystine dependent dwarf colony coliforms. This medium is recommended for use in urinary bacteriology, promoting the growth of all urinary pathogens. C.L.E.D. Medium is also recommended for dip stick procedures and as dip inoculum transport medium for urine specimens.
Bacteriuria may be quantitated by inoculating the surface of an agar medium by proper dilution. Inoculate the medium immediately after urine collection. It can also be inoculated by calibrated loop or duplicate dilution pour plate methods.

for isolation and di?erentiation of urinary pathogens by lactose fermentation