CHOPPED LIVER BROTH (CL BROTH)

Clostridium species are one of the major causes of food poisoning and gastrointestinal illnesses. They are gram-positive, spore-forming rods that occur naturally in soil. Among the family are: Clostridium botulinum, which produces one of the most potent toxins in existence; Clostridium tetani, causative agent of tetanus; and Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damage host cells is a method deployed by many bacterial pathogens. The major virulence factor of C. perfringens is the CPE enterotoxin, which is secreted Recommended for the cultivation and enrichment of anaerobic bacteria from food specimen. Chopped Liver Broth is formulated in accordance with APHA and is recommended by FDA for cultivation and enrichment of Clostridium species from foods.
Liquid foods are directly inoculated whereas solid foods are grinded first and then inoculated into this enrichment medium. 1 to 2 grams of solid or 1 to 2 ml of liquid food is added per 15 ml of enrichment broth and incubation is carried out at 26 to 28?C for upto 7 days. If no growth is observed after 7 days the culture medium is further incubated for 10 days to allow delayed germination of spores.

for the cultivation and enrichment of anaerobic bacteria from food specimen