CHROMOGENIC E. COLI AGAR

Chromogenic E.coli Agar is based on Tryptone Bile Agar to detect Escherichia coli in foods, where recovery of E.coli is faster, more reliable and accurate. Most of the E.coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase, which is highly specific for E.coli. The chromogenic agent X-glucuronide used in this medium helps to detect glucuronidase activity of E.coli. E.coli cells absorb X-glucuronide and the intracellular glucuronidase enzyme splits the bond between the chromophore and the glucuronide. The released chromophore gives bluish green coloration to the E.coli colonies. This medium is recommended for isolation of E.coli from water, food and clinical samples.

for the detection and enumeration of Escherichia coli in foods without further con?rmation on membrane ?lter or by indole reagent